5-Amino-1MQ: Research Profile and Protocol Guide

Overview

5-Amino-1MQ is a small molecule inhibitor of nicotinamide N-methyltransferase (NNMT), an enzyme involved in cellular energy metabolism and fat storage. While technically not a peptide, it is commonly categorized alongside metabolic peptides in the research supplier market due to its overlapping research applications. NNMT is overexpressed in adipose tissue of obese individuals, and its inhibition has been shown to increase NAD+ levels, enhance cellular energy expenditure, and reduce lipid accumulation in preclinical models. Research published in Biochemical Pharmacology demonstrated that 5-Amino-1MQ treatment reduced body weight, white adipose tissue mass, and adipocyte size in diet-induced obesity mouse models without affecting food intake - suggesting a direct metabolic mechanism rather than appetite suppression.

Mechanism of Action

5-Amino-1MQ works by inhibiting NNMT, which normally methylates nicotinamide (a form of vitamin B3), consuming methyl donors and diverting nicotinamide away from the NAD+ salvage pathway. By blocking NNMT, 5-Amino-1MQ increases intracellular NAD+ availability, which activates sirtuins and other NAD+-dependent metabolic enzymes. This leads to enhanced mitochondrial function, increased fatty acid oxidation, and reduced lipogenesis in adipocytes. Additionally, NNMT inhibition has been shown to alter the epigenetic landscape of fat cells through changes in methyl donor availability, potentially reprogramming adipocyte metabolism toward a more metabolically active state.

Research Applications

Obesity and metabolic research (primary), NAD+ pathway modulation, adipocyte biology, epigenetic metabolism studies, potential synergy with NAD+ precursors (NMN/NR)

Research Protocols

Safety Profile and Considerations

Limited to preclinical data. No significant toxicity reported in rodent studies at standard research doses. No human clinical trials published. Not FDA approved. As a small molecule rather than peptide, pharmacokinetic profile differs from typical peptide compounds.

Storage and Stability

Room temperature, protect from moisture and light. Typically supplied as powder or capsule. See our storage and handling guide for comprehensive best practices.

Published Research

• Neelakantan H, et al. Selective and membrane-permeable small molecule inhibitors of NNMT. Biochem Pharmacol. 2017
  PMID: 29180275
• Pissios P. NNMT: A Master Metabolic Regulator. Trends Endocrinol Metab. 2017
  PMID: 28456432
• Kraus D, et al. NNMT knockdown in adipose tissue. Nature. 2014
  PMID: 24670636

Search for additional 5-Amino-1MQ research on PubMed.

Where to Source 5-Amino-1MQ

Peptide purity is critical for research accuracy. We evaluate suppliers against our 100-point methodology.